نوع مقاله : پژوهشی- فارسی
نویسندگان
1 گروه میکروبیولوژی، دانشکده علوم زیستی، دانشگاه الزهرا ، تهران ، ایران
2 گروه میکروبیولوژی ، دانشکده علوم زیستی، دانشگاه الزهرا، تهران، ایران.
3 گروه باکتری شناسی، انستیتوپاستور ایران، تهران، ایران،
4 دانشکده علوم پایه و فناوری های نوین زیستی، دانشگاه علم و فرهنگ، تهران، ایران
5 گروه باکتری شناسی، انستیتوپاستور ایران، تهران، ایران
6 گروه باکتری شناسی، انیستیتو پاستور ایران، تهران، ایران.
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Introduction: Staphylococcus aureus, especially Methicillin-Resistant Staphylococcus aureu, (MRSA) is one of the major causes of nosocomial and community-acquired infections. Nowadays, it is known as a health problem in the world due to its resistance to most antibiotics. The present study aimed to determine the prevalence of the vanA gene in MRSA isolated from patients with bedsores.
Materials and Methods: In the current study, 80 clinical isolates were collected from patients with bedsores at Loghman-e Hakim Hospital, Iranmehr Hospital, and Tehran Wound Center. The samples were identified using standard laboratory tests and resistance to cefoxitin disk (30µg) and evaluating the presence of the mecA gene. The resistance of the MRSA isolates to different antibiotics was checked using the disc diffusion method. The presence of the vanA gene in these isolates was investigated using specific primers and polymerase chain reaction (PCR).
Results: Of the studied isolates, 29 isolates were identified as methicillin-resistant. The highest antibiotic resistance level was observed against erythromycin and clindamycin. The lowest antibiotic resistance level was determined against linezolid with 82.75%, 82.75%, and 13.8% respectively. The vanA gene was detected in all of the MRSA isolates. Also, multidrug resistance (MDR) was observed in almost 72.41% of isolates.
Discussion and Conclusion: The prevalence of the vanA gene has increased in MRSA isolates. In this regard, high and multiple isolates of Staphylococcus aureus resistant to common antibiotics should be considered a serious matter.
کلیدواژهها [English]
References