نوع مقاله : پژوهشی- فارسی
نویسندگان
1 گروه میکروبیولوژی، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران
2 گروه زیست شناسی، واحد ارسنجان، دانشگاه آزاد اسلامی، ارسنجان، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Introduction: Probiotic bacteria, including lactobacilli, are known to prevent many cancers. This study aimed to evaluate the anti-cancer effects of Lactobacillus cytoplasmic extract isolated from dairy products in Guilan province on HT-29 colon cancer cell line.
Materials and methods: In this study, Lactobacillus bacteria were isolated and identified from dairy products based on conventional culture and biochemical methods. Then, using specific primers, the 16S rRNA gene of the bacteria was amplified and the confirmed samples were compared with the sequences in NCBI after sequencing. Different concentrations of 0.5, 0.75, 1, 1.5, and 2 mg/mL were made from the cytoplasmic extract of the selected strain. Finally, the cytotoxicity effects of Lactobacillus cytoplasmic extract on cancer HT-29 and normal HUVEC cell lines were investigated by the MTT method.
Results: The results of the MTT assay showed that the cytoplasmic extract of Lactobacillus dose-dependently, reduced the survival and proliferation of colon cancer cells at 24 h, with the highest inhibitory effect at 2 mg/ml. Concentrations of 1, 1.5, and 2 mg/mL decreased cell viability by 50±4.87%, 40±8.61%, and 25±5.80%, respectively. The IC50 value of the cytoplasmic extract was 1.43 mg/mL for the HT29 cells. Also, cytotoxic effects, on the normal HUVEC cell line were not observed.
Discussion and conclusion: According to the results, the cytoplasmic extract of Lactobacillus isolated from dairy products reduced the growth of HT-29 cancer cells, which with further studies can be used as a biological product in the treatment and prevention of colon cancer.
کلیدواژهها [English]
)