نوع مقاله : پژوهشی- انگلیسی
نویسندگان
1 استاد بیولوژی و بیوتکنولوژی خاک، دانشکده کشاورزی، دانشگاه تبریز، ایران
2 گروه علوم و مهندسی خاک، دانشکده کشورزی، دانشگاه تبریز
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Soil contamination with petroleum hydrocarbons is a major environmental challenge in oil-producing regions, including the Persian Gulf. Conventional remediation methods are costly and may cause secondary pollution, highlighting the need for sustainable biological alternatives. This study aimed to isolate indigenous hydrocarbon-degrading bacteria from oil-contaminated soil of Kuwait (initial total petroleum hydrocarbons, TPH, 10%) and evaluate their bioremediation potential. Enrichment in minimal salt medium with crude oil (2% v/v) as the sole carbon source yielded 11 bacterial isolates (KK3, KK4, KK6, KK7, KK8, KK9, KK10, KK14, KK16, KK17, KK19). Their performance was compared with two reference strains (Pseudomonas sp. S21-1 and H712) in a 60-day microcosm experiment (completely randomized design, 14 treatments, triplicate) using naturally contaminated soil. TPH reduction was measured gravimetrically after Soxhlet extraction. Isolate KK17 achieved the highest degradation (38%), significantly outperforming reference strains (e.g., S21-1: 21.5%). However, KK17 grew poorly at 50°C, limiting its use in hot climates. In contrast, KK16 and KK14 showed lower degradation (29% and 28%, respectively) but grew well at 50°C, making them more suitable for field applications in the Persian Gulf region. Qualitative observations (soil color lightening, reduced odor) supported the quantitative findings. Although KK17 was the most effective degrader at moderate temperature, KK16 and KK14 are better candidates for field-scale bioremediation due to their thermotolerance. Future work should focus on molecular characterization, process optimization, and consortium development.
کلیدواژهها [English]
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