دانشگاه اصفهان زیست شناسی میکروبی 3060-7647 14 56 2026 01 06 The rate of IFNL3 and MTTP genes polymorphism in β-thalassemia major patients infected with hepatitis C virus treated with ledipasvir-sofosbuvir The rate of IFNL3 and MTTP genes polymorphism in β-thalassemia major patients infected with hepatitis C virus treated with ledipasvir-sofosbuvir 43 57 29954 10.22108/bjm.2025.146169.1645 FA Hussein Sattar Abbood Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran. Majid Bouzari Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Hezar Jereeb Street, 81746-73441, Isfahan, Iran Abbas Soleimani-Delfan Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran. Hussam Sami Awayid Middle Technical University, Department of Medical Laboratory, Institute of Technical-Suwaira, Iraq. Journal Article 2025 07 30 The main goal of the present study was to investigate the prevalence of interferon lambda 3 (IFNL3) and microsomal triglyceride transfer protein (MTTP) encoding gene polymorphism among thalassemia patients infected with HCV. The frequency of IFNL3 and MTTP encoding gene polymorphism in 79 thalassemia patients infected with HCV and their correlation with the treatment outcome using ledipasvir/sofosbuvir was investigated. Single nucleotide polymorphism detection confirmed the GT, TT, and GG (rs1800591) polymorphism of the synthesized MTTP gene fragment and AT, AA, and TT (rs8113007) and TG, TT, and GG (rs8099917) polymorphisms of the synthesized IFNL3 gene fragment. GG was the most frequent allele of rs1800591 in both males and females followed by GT allele which was 11.39% in males and 19% in females. The TT allele of rs8099917 was the most prevalent (37% in females and 25% in males), and the GG allele was the least frequent. Also, the AT allele was more prevalent than the AA and TT alleles of rs8113007. No significant correlation was observed between the SNPs detected and the treatment outcome. The GG allele of the MTTP gene was the most effective allele involved in decreasing the ALT, AST, and ALP enzymes. For IFNL3 (rs8099917) and IFNL3 (rs8113007), the TT and AT alleles were the most effective alleles for the ALP enzyme levels, respectively. It seems that there is a correlation between the frequent alleles detected and liver enzymes. The main goal of the present study was to investigate the prevalence of interferon lambda 3 (IFNL3) and microsomal triglyceride transfer protein (MTTP) encoding gene polymorphism among thalassemia patients infected with HCV. The frequency of IFNL3 and MTTP encoding gene polymorphism in 79 thalassemia patients infected with HCV and their correlation with the treatment outcome using ledipasvir/sofosbuvir was investigated. Single nucleotide polymorphism detection confirmed the GT, TT, and GG (rs1800591) polymorphism of the synthesized MTTP gene fragment and AT, AA, and TT (rs8113007) and TG, TT, and GG (rs8099917) polymorphisms of the synthesized IFNL3 gene fragment. GG was the most frequent allele of rs1800591 in both males and females followed by GT allele which was 11.39% in males and 19% in females. The TT allele of rs8099917 was the most prevalent (37% in females and 25% in males), and the GG allele was the least frequent. Also, the AT allele was more prevalent than the AA and TT alleles of rs8113007. No significant correlation was observed between the SNPs detected and the treatment outcome. The GG allele of the MTTP gene was the most effective allele involved in decreasing the ALT, AST, and ALP enzymes. For IFNL3 (rs8099917) and IFNL3 (rs8113007), the TT and AT alleles were the most effective alleles for the ALP enzyme levels, respectively. It seems that there is a correlation between the frequent alleles detected and liver enzymes. https://bjm.ui.ac.ir/article_29954_528bbf4a6f68a5868f37f5cf8d8e7c25.pdf