University of Isfahan Journal of Microbial Biology 3060-7647 8 32 2019 12 22 The Effects of using Chemical Fertilizers along with Guillard (f/2) on Growth, Biochemical Compositions, Fatty Acid Profiles, Chlorophylls and Total Carotenoids of Haematococcus pluvialis in Brackish Water The Effects of using Chemical Fertilizers along with Guillard (f/2) on Growth, Biochemical Compositions, Fatty Acid Profiles, Chlorophylls and Total Carotenoids of Haematococcus pluvialis in Brackish Water 65 79 23164 10.22108/bjm.2018.112651.1155 FA Saeid Vahdat Ph.D student, Department of Biology and Aquaculture, Faculty of Artemia and Aquaculture Institute, Urmia University, Urmia, Iran Manizheh Biabani Asrami Ph.D student, Department of Fisheries, Faculty of Environment and fishery, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran Behrooz Atashbar Assistant Professor, Department of Ecology, Faculty of Urmia Lake Research Institute, Urmia University, Urmia, Iran. Farzaneh Noori Assistant Professor, Department of Biology and Aquaculture, Faculty of Artemia and Aquaculture Institute, Urmia University, Urmia, Iran Journal Article 2018 09 10 Introduction: Using the standard methods for the productions of unicellular algae have high harvest costs and in many cases is not affordable. Therefore, chemical fertilizers could be considered as an appropriate alternative for the production of unicellular algae. The green microalgae Haematococcus pluvialis is capable of producing high levels of astaxanthin and lipid in resistant cells called spore. Materials and methods: H. pluvialis with the initial density of 4.25 × 104 cell mL-1 transferred to the 3-liter containers. Fatty acid profiles, growth factors, chemical compositions and pigments of algae were investigated. Results: The results showed that the specific growth rate, the cell division per day, carbon dioxide fixation efficiency, biomass, and total biomass efficiency were significantly higher and lower in treatment 5 (G: I= 50:50) and 9 (Gf= 100), respectively. The highest protein (40.68%), lipid (34.88%) and carbohydrate (42.04%) contents were observed in treatments of 9, 2 (G: R= 50:50) and 8 (I= 100), respectively. The highest chlorophyll a (71.29 mg/g FW) in T9 and chlorophyll b (85.60 mg/g FW) in T3 (G: R= 75:25) and total carotenoid (206.11 mg/g FW) in T6 (G: R= 75:25). Saturated fatty acid was significantly highest in T6, but its poly unsaturated fatty acid was significantly highest in T2. Discussion and conclusion: The results indicated that the f/2 medium promotes biomass and lipid in T9 and T5, T6 made increase total carotene and SFA and T2 and T4 raised Mono saturated fatty acid and PUFA in H. pluvialis when it is cultured in brackish water. Introduction: Using the standard methods for the productions of unicellular algae have high harvest costs and in many cases is not affordable. Therefore, chemical fertilizers could be considered as an appropriate alternative for the production of unicellular algae. The green microalgae Haematococcus pluvialis is capable of producing high levels of astaxanthin and lipid in resistant cells called spore. Materials and methods: H. pluvialis with the initial density of 4.25 × 104 cell mL-1 transferred to the 3-liter containers. Fatty acid profiles, growth factors, chemical compositions and pigments of algae were investigated. Results: The results showed that the specific growth rate, the cell division per day, carbon dioxide fixation efficiency, biomass, and total biomass efficiency were significantly higher and lower in treatment 5 (G: I= 50:50) and 9 (Gf= 100), respectively. The highest protein (40.68%), lipid (34.88%) and carbohydrate (42.04%) contents were observed in treatments of 9, 2 (G: R= 50:50) and 8 (I= 100), respectively. The highest chlorophyll a (71.29 mg/g FW) in T9 and chlorophyll b (85.60 mg/g FW) in T3 (G: R= 75:25) and total carotenoid (206.11 mg/g FW) in T6 (G: R= 75:25). Saturated fatty acid was significantly highest in T6, but its poly unsaturated fatty acid was significantly highest in T2. Discussion and conclusion: The results indicated that the f/2 medium promotes biomass and lipid in T9 and T5, T6 made increase total carotene and SFA and T2 and T4 raised Mono saturated fatty acid and PUFA in H. pluvialis when it is cultured in brackish water.

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