University of Isfahan Journal of Microbial Biology 3060-7647 6 23 2017 09 23 FMD virus type Asia-1 irradiated vaccine and evaluation of immune response on guinea-pig model FMD virus type Asia-1 irradiated vaccine and evaluation of immune response on guinea-pig model 57 66 21662 10.22108/bjm.2017.21662 FA Farahnaz Motamedi-Sedeh Associate professor of Virology, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Tehran, Iran Homayoon Mahravani Associate professor of Microbiology, Razi Vaccine and Serum Research Institute, Karaj, Iran Mojgan Javadi M.Sc. student of Microbiology, Islamic Azad University, Karaj Branch, Iran Naser Harzandi Assistant professor of Microbiology, Islamic Azad University, Karaj Branch, Iran Sayed Kamalodin Shafaei Nuclear Agriculture Research Center, Institute of Nuclear Science and Technology, Iran Journal Article 2016 02 13 <strong>Introduction:</strong> Foot and Mouth Disease (FMD) is the most contagious disease in cloven-hoofed animals which causes a lot of economical losses. FMD virus belongs to Picornaviridae family and Aphthovirus genus. The aim of the study is evaluation of humoral and cellular immune responses triggered by a Gamma-irradiated FMD vaccine in the guinea-pig model. <br /><strong>Materials and Methods:</strong> FMD virus type Asia-1 was multiplied on BHK₂₁ cell line and irradiated by gamma ray in different doses. According  to dose/survival curve, D₁₀value and optimum dose of virus inactivation were calculated 7.69 and 50 kGy, respectively. Antigenic characteristic of irradiated and un-irradiated virus samples were evaluated by complement fixation test (CF test) and safety test was done by four blind passages cell culture on IBRS₂ cell line. The inactivated virus sample was formulated as Radio- vaccine and immune responses were evaluated in three groups of ginea-pigs; the first group Radio-vaccine, the second group conventional vaccine and the last one was negative control. <br /><strong>Results:</strong> The results of neutralizing antibody titration for two vaccinated groups were significant (P<0.05), but between Radio-vaccine and conventional vaccine was no significant. The increase in splenocyte multiplication for two vaccinated groups by MTT test was significant (P<0.05). <br /><strong>Discussion and conclusion:</strong> The Gamma irradiated inactivated FMD type Asia-1 vaccine is a good candidate for immunization of guinea-pig without the problems such as residue, virus escape and etc. And it can use for vaccination of cattle in the future. <strong>Introduction:</strong> Foot and Mouth Disease (FMD) is the most contagious disease in cloven-hoofed animals which causes a lot of economical losses. FMD virus belongs to Picornaviridae family and Aphthovirus genus. The aim of the study is evaluation of humoral and cellular immune responses triggered by a Gamma-irradiated FMD vaccine in the guinea-pig model. <br /><strong>Materials and Methods:</strong> FMD virus type Asia-1 was multiplied on BHK₂₁ cell line and irradiated by gamma ray in different doses. According  to dose/survival curve, D₁₀value and optimum dose of virus inactivation were calculated 7.69 and 50 kGy, respectively. Antigenic characteristic of irradiated and un-irradiated virus samples were evaluated by complement fixation test (CF test) and safety test was done by four blind passages cell culture on IBRS₂ cell line. The inactivated virus sample was formulated as Radio- vaccine and immune responses were evaluated in three groups of ginea-pigs; the first group Radio-vaccine, the second group conventional vaccine and the last one was negative control. <br /><strong>Results:</strong> The results of neutralizing antibody titration for two vaccinated groups were significant (P<0.05), but between Radio-vaccine and conventional vaccine was no significant. The increase in splenocyte multiplication for two vaccinated groups by MTT test was significant (P<0.05). <br /><strong>Discussion and conclusion:</strong> The Gamma irradiated inactivated FMD type Asia-1 vaccine is a good candidate for immunization of guinea-pig without the problems such as residue, virus escape and etc. And it can use for vaccination of cattle in the future. https://bjm.ui.ac.ir/article_21662_8f2b98220547f6ee41cea4015ec6c585.pdf