University of Isfahan Journal of Microbial Biology 3060-7647 6 22 2017 06 22 Evaluation of Interferon-gamma Application for Recognition of Patients Afflicted by Non-healing Cutaneous Leishmaniasis Evaluation of Interferon-gamma Application for Recognition of Patients Afflicted by Non-healing Cutaneous Leishmaniasis 101 112 21560 10.22108/bjm.2017.21560 FA Mohammad Moafi PhD Student of Immunology, Bu-Ali Sina University, Hamedan, Iran Hossein Rezvan PhD in Immunoparasitology, Bu-Ali Sina University, Hamedan, Iran Roya Sherkat Associate Professor of Infectious Diseases and Tropical Medicine, Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Iran Sayyed Hamid Zarkesh-Esfahani Associate Professor of Immunology, University of Isfahan, Iran Roya Taleban Community Medicine Specialist, Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Ali Asilian Professor of Dermatology, Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Iran Mohammad Ali Nilforoushzadeh Professor of Dermatology, Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran Fariba Jaffary Professor of Pharmacology, Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Marjan Mansourian Associate Professor of Biostatistics, Isfahan University of Medical Sciences, Isfahan, Iran Fatemeh Sokhanvari General Physician, Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Nazli Ansari General Physician, Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran Journal Article 2016 11 08 Introduction:Different studies undertaken in the animal modeling show that Interferon-gamma deficiency impairs healing process of Leishmania infection. It seems that the level of Interferon-gamma production could also affect the healing duration of Leishmania lesion in humans. The current study aims to investigate the possibility of Interferon-gamma application for recognition of cases afflicted by non-healing Leishmaniasis. Materials and methods: Peripheral blood mononuclear cells (PBMCs) of 32 patients, who were afflicted by healing or non-healing Leishmaniasis, were isolated and the levels of interferon-gamma were determined, using ELISA method. Afterwards, the cut-off point of interferon-gamma to identify patients afflicted by non-healing Leishmaniasis was calculated through ROC-Curve analysis. Furthermore, Leishmanin Skin Test (LST) was performed for every patient. Results: Levels of Interferon-gamma produced by PBMCs stimulated with Soluble Leishmania Antigen (SLA) or Phytohemaglotinine were significantly higher in healing patients, compared with non-healing individuals (p <0.01). Interferon-gamma cut-off point in the optimized sensitivity (87.5%) and specificity (100%) was 1208 pg/ml. In addition, Levels of induration resulted from Lesihmanin Skin Test (LST) was significantly higher in healing patients, compared with healing individuals (p=0.023). Discussion and conclusion: Interferon-gamma deficiency could be considered as one of the causative factors in non-healing Leishmaniasis in humans. In fact, Interferon-gamma deficiency can identify some patients afflicted by non-healing Leishmaniasis. Introduction:Different studies undertaken in the animal modeling show that Interferon-gamma deficiency impairs healing process of Leishmania infection. It seems that the level of Interferon-gamma production could also affect the healing duration of Leishmania lesion in humans. The current study aims to investigate the possibility of Interferon-gamma application for recognition of cases afflicted by non-healing Leishmaniasis. Materials and methods: Peripheral blood mononuclear cells (PBMCs) of 32 patients, who were afflicted by healing or non-healing Leishmaniasis, were isolated and the levels of interferon-gamma were determined, using ELISA method. Afterwards, the cut-off point of interferon-gamma to identify patients afflicted by non-healing Leishmaniasis was calculated through ROC-Curve analysis. Furthermore, Leishmanin Skin Test (LST) was performed for every patient. Results: Levels of Interferon-gamma produced by PBMCs stimulated with Soluble Leishmania Antigen (SLA) or Phytohemaglotinine were significantly higher in healing patients, compared with non-healing individuals (p <0.01). Interferon-gamma cut-off point in the optimized sensitivity (87.5%) and specificity (100%) was 1208 pg/ml. In addition, Levels of induration resulted from Lesihmanin Skin Test (LST) was significantly higher in healing patients, compared with healing individuals (p=0.023). Discussion and conclusion: Interferon-gamma deficiency could be considered as one of the causative factors in non-healing Leishmaniasis in humans. In fact, Interferon-gamma deficiency can identify some patients afflicted by non-healing Leishmaniasis.

https://bjm.ui.ac.ir/article_21560_2051a862c2eb78a7b0b9bdebc2a4199b.pdf