University of Isfahan Journal of Microbial Biology 3060-7647 5 17 2018 04 08 Isolation and identification of the acetonitrile-degrading Pseudomonas from the Ab-anbar-e Ganjali Khan in Kerman Isolation and identification of the acetonitrile-degrading Pseudomonas from the Ab-anbar-e Ganjali Khan in Kerman 73 86 20370 10.22108/bjm.2016.20370 FA Narjes Ramezanipour M.Sc. of Microbiology, Department of Biology, Faculty of sciences, Shahid Bahonar University of Kerman, Kerman, Iran Arastoo Badouei-Dalfard Associate Professors of Biochemistry, Department of Biology, Faculty of sciences, Shahid Bahonar University of Kerman, Kerman, Iran Abdolhamid Namaki-Shoushtari Associate Professors of Genetics, Department of Biology, Faculty of sciences, Shahid Bahonar University of Kerman, Kerman, Iran Zahra Karami Assistant Professors of Biophysics, Department of Biology, Faculty of sciences, Shahid Bahonar University of Kerman, Kerman, Iran Journal Article 2018 04 08 Introduction: Nitriles are toxic and hazardous compounds for all organisms which are produced enormously by human being and cause environment pollution. Biodegradation is the best method for Nitrile elimination in sewage. The aim of this study was isolation and identification of native bacteria Acetonitrile-degrading from the sewage of the city of Kerman. <br /> <br />Materials and methods: The enrichment and screening of Acetonitrile degrading bacteria was performed in a specific medium containing 1 % Acetonitrile as sole carbon and nitrogen source. Enzyme activity and ammonium production was determined in growth medium using a modification of the phenol/hypochlorite method. Identification of the isolates was undertaken using microbial - biochemical and molecular tests. The optimization of enzyme production was examined. The amount of nitrile and acid were also determined by gas chromatography. <br /> <br />Results: Among three isolated nitrile hydrolyzing producing species (FA3, FA8 and AB19), FA8 isolate showed maximum enzyme productivity. The results of characteristic tests showed that these bacteria belonged to the Pseudomonas sp. Moreover, 16S rRNA sequencing and phylogenetic analyses exhibited that FA11, FA8 and AB19 strains were similar to Pseudomonas Otitidis and Pseudomonas geniculate with 99, 100% homology, respectively. Results of medium optimization of Pseudomonas FA8 strain showed that glucose (10 gL-1) and yeast extract (5 gL-1) in neutral medium strongly supported enzyme production. Gas chromatography results showed that FA8 produced 58% acetic acid at 48 h of incubation. <br /> <br />Discussion and conclusion: The results demonstrated that Pseudomonas FA8 isolated bacterium is a suitable candidate for degradation of Acetonitrile. This bacterium could be used for treatment of industrial wastewater and sites that contaminated with Acetonitrile. Introduction: Nitriles are toxic and hazardous compounds for all organisms which are produced enormously by human being and cause environment pollution. Biodegradation is the best method for Nitrile elimination in sewage. The aim of this study was isolation and identification of native bacteria Acetonitrile-degrading from the sewage of the city of Kerman. <br /> <br />Materials and methods: The enrichment and screening of Acetonitrile degrading bacteria was performed in a specific medium containing 1 % Acetonitrile as sole carbon and nitrogen source. Enzyme activity and ammonium production was determined in growth medium using a modification of the phenol/hypochlorite method. Identification of the isolates was undertaken using microbial - biochemical and molecular tests. The optimization of enzyme production was examined. The amount of nitrile and acid were also determined by gas chromatography. <br /> <br />Results: Among three isolated nitrile hydrolyzing producing species (FA3, FA8 and AB19), FA8 isolate showed maximum enzyme productivity. The results of characteristic tests showed that these bacteria belonged to the Pseudomonas sp. Moreover, 16S rRNA sequencing and phylogenetic analyses exhibited that FA11, FA8 and AB19 strains were similar to Pseudomonas Otitidis and Pseudomonas geniculate with 99, 100% homology, respectively. Results of medium optimization of Pseudomonas FA8 strain showed that glucose (10 gL-1) and yeast extract (5 gL-1) in neutral medium strongly supported enzyme production. Gas chromatography results showed that FA8 produced 58% acetic acid at 48 h of incubation. <br /> <br />Discussion and conclusion: The results demonstrated that Pseudomonas FA8 isolated bacterium is a suitable candidate for degradation of Acetonitrile. This bacterium could be used for treatment of industrial wastewater and sites that contaminated with Acetonitrile. https://bjm.ui.ac.ir/article_20370_d6f4689dbed814cf26a25d3080cd15c2.pdf