University of Isfahan Journal of Microbial Biology 3060-7647 3 9 2014 05 22 Molecular detection of Chlamydophila felis in ocular discharge of domestic cats in Tehran & Isfahan Molecular detection of Chlamydophila felis in ocular discharge of domestic cats in Tehran & Isfahan 45 52 19518 FA Hassan Momtaz Associated Professor of Microbiology,Shahrekord Branch, Islamic Azad University, Iran Mohammad Alirezaie Graduated of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Iran Peyman Keyhani Assistant Professor of Small Animal Internal Medicine, Shahrekord Branch, Islamic Azad University, Iran Journal Article 2016 06 14   Introduction : Chlamydophila felis is one of the most common pathogens isolated from ocular discharges in domestic cats. The aim of the present study was to detect this microorganism in ocular discharges collected from domestic cats in Tehran and Isfahan cities   Materials and methods: From a total of 224 domestic cats, ocular swabs were collected and DNA was extracted. Samples were studied using the Polymerase Chain Reaction method and their products were analyzed using agarose gel .   Results : From a total of 224 samples studied, 40 samples (17.85%) were infected with Chlamydophila felis which were diagnosed as positive in PCR test .   Discussion and conclusion : According to the difficulties and time-consuming process of isolation of Chlamydophila felis in laboratory culture media, the PCR technique has been suggested as a rapid and safe method for detection of this organism in clinical samples .   Introduction : Chlamydophila felis is one of the most common pathogens isolated from ocular discharges in domestic cats. The aim of the present study was to detect this microorganism in ocular discharges collected from domestic cats in Tehran and Isfahan cities   Materials and methods: From a total of 224 domestic cats, ocular swabs were collected and DNA was extracted. Samples were studied using the Polymerase Chain Reaction method and their products were analyzed using agarose gel .   Results : From a total of 224 samples studied, 40 samples (17.85%) were infected with Chlamydophila felis which were diagnosed as positive in PCR test .   Discussion and conclusion : According to the difficulties and time-consuming process of isolation of Chlamydophila felis in laboratory culture media, the PCR technique has been suggested as a rapid and safe method for detection of this organism in clinical samples . https://bjm.ui.ac.ir/article_19518_8f07c42582668b2cb5005d3487a5ace7.pdf